Table 2.
Stoichiometries and equilibrium constants for binding to normal and 6mG-containing DNAs
| DNA | Protein | Stoichiometry (n) |
Association constants |
|||
|---|---|---|---|---|---|---|
| 1st step | 2nd step | K1 | K2 | K1/K2 | ||
| Unmodified ds-26mer | Wild-type | 5.9 ± 0.5 | – | 9.1 ± 4.2 × 105 M−1 | – | – |
| Unmodified ds-26mer | C145S | 6.1 ± 0.3 | – | 2.2 ± 1.1 × 106 M−1 | – | – |
| 6mG ds-26mer | P140K | 2.3 ± 0.3 | 4.1 ± 0.3 | 1.7 ± 0.3 × 107 M−1 | 3.4 ± 0.8 × 106 M−1 | 5.0 ± 0.3 |
| 6mG ds-26mer | C145S | 2.0 ± 0.2 | 3.4 ± 0.1 | 6.2 ± 2.4 × 106 M−1 | 1.9 ± 0.4 × 106 M−1 | 3.3 ± 0.4 |
| 6mG ds-24mer | C145S | 1.8 ± 0.1 | 2.1 ± 0.1 | 2.5 ± 1.2 × 107 M−1 | 4.4 ± 2.8 × 106 M−1 | 5.7 ± 0.8 |
| Unmodified ss-26mer | C145S | 5.7 ± 0.4 | – | 2.2 ± 0.3 × 106 M−1 | – | – |
| 6mG ss-26mer | C145S | 6.3 ± 0.5 | – | 4.5 ± 0.5 × 106 M−1 | – | – |