Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jul 13;40(18):9036–9043. doi: 10.1093/nar/gks675

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Molecular characterization of blue and white sectors taken from lesion-containing sectored colonies. The sequence around the G-AAF adduct site shows the local heteroduplex that allows strand discrimination following replication. The non-damaged strand carries a 3 nt insertion that will generate an additional NlaIII restriction site during replication. Two PCR primers were designed to amplify a 610-bp fragment around the lesion site. The figure represents the analysis of individual sectors from seven sectored clones resulting from the integration of an AAF damaged vector (Figure 2B). Analysis of the white sectors (bottom gel) by NlaIII digestion shows the presence of an additional NlaIII restriction site corresponding to the replication of the non-damaged strand of the chromosome. Analysis of the blue sectors (top gel) lacks the additional NlaIII site; sequencing of these PCR fragments showed the expected sequence resulting from TLS events on the damaged strand (i.e. frameshift-2 in the NarI hotspot where the lesion is located (23).