Table 2.

Primers for site-directed mutagenesis

Primer	Sequence
W131A-forward	5′-GTCTGGCTCGTCTGGCGCCGGATGGTCTGC-3′
W131A-reverse	5′-GCAGACCATCCGGCGCCAGACGAGCCAGAC-3′
S73A-forward	5′-CATGCCGCAAATGCAGCCGCAGAT-3′
S73A-reverse	5′-ATCTGCGGCTGCATTTGCGGCATG-3′
T159A-forward	5′-GTTTTTCGACGGCGCCGCGCTGCACGCACTG-3′
T159A-reverse	5′-CAGTGCGTGCAGCGCGGCGCCGTCGAAAAAC-3′
Y101A-forward	5′-CGTCTGACCACGGTCGCACTGGCAGATCAGGAC-3′
Y101A-reverse	5′-GTCCTGATCTGCCAGTGCGACCGTGGTCAGACG-3′
Y115A-forward	5′-CACCCTGAACGAACTGGCAATGGCGGCCGCACATC-3′
Y115A-reverse	5′-GATGTGCGGCCGCCATTGCCAGTTCGTTCAGGGTG-3′
W80A-forward	5′-CTGCTGGCCCAGGCGCGTTCTGATCTG-3′
W80A-reverse	5′-CAGATCAGAACGCGCCTGGGCCAGCAG-3′
D156A-forward	5′-GTCACCGTGTTTTTCGCAGGCGCCACGCTGCAC-3′
D156A-reverse	5′-GTGCAGCGTGGCGCCTGCGAAAAACACGGTGAC-3′
N112A-forward	5′-CGTTACCGCACCCTGGCAGAACTGTATATGGC-3′
N112A-reverse	5′-GCCATATACAGTTCTGCCAGGGTGCGGTAACG-3′