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. 2012 Jul 22;40(18):9182–9192. doi: 10.1093/nar/gks683

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — CBNAC interacts with SNI1. (A) Yeast two-hybrid analysis. Transformants of yeast strain pJ69-4A were grown as indicated (upper left) on minimal medium with (+Ade) or without (–Ade) selection. Adenine prototrophy indicates positive interaction. β-Galactosidase activity in the colonies grown in +Ade medium was determined by filter-lift assay (LacZ). (B) LCI assay for detecting interaction in planta. Tobacco leaves were transformed by Agrobacterium infiltration using a needleless syringe. The indicated NLuc and CLuc construct pairs were used for transformation. Shown are luminescence images (upper panel) and quantitative luminescence measurements (lower panel) depicting luciferase activity in inoculated leaves at 48 hpi.