Figure 5.

Altered responses of the cbnac1 sni1 double mutant to PstDC3000. (A) Morphology of 5-week-old wild-type (WT), cbnac1, sni1 and cbnac1 sni1 plants grown on MS agar plates. (B–D) Disease resistance responses in leaves inoculated with bacterial suspension as in Figure 3. Disease symptoms in inoculated leaves at 5 dpi are depicted (B). Bacterial growth in inoculated leaves at 0 and 3 dpi are compared (C). Mean bacterial densities ± SE were calculated from six to eight replicate plants. Significant differences as calculated by Student’s t test (P < 0.05) are indicated by unique letters. The experiment was repeated at least three times with similar results. PR1 expression was monitored in inoculated leaves by qRT-PCR (D). Values were normalized using the expression level of Tubulin 2 and expressed relative to the expression level in WT at 12hpi, which is arbitrarily set at 100. Mean relative expression values ±SE values from three independent experiments are shown. Data were analyzed by Student’s t test. Different letters indicate statistically significant differences between genotypes (P < 0.05).