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Direct sequencing of the RT-PCR product and sequence alignment revealed a break at nucleotide 1279 in BCR (nucleotides highlighted in blue) and at nucleotide 2434 in JAK2 (nucleotides highlighted in red and italicized); the fusion product included the entire exon 1 of BCR fused to nt 1 of exon 19 of JAK2 in frame.
