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. 2012 May 22;2:12. doi: 10.1186/2043-9113-2-12

Figure 1.

Figure 1

Western blotting analysis of hazelnut processed by AC and HHP treatments. Western blotting analysis of AC/HHP flour hazelnut samples incubated with clinical sera from patients allergic to hazelnut (*) Figure2 is illustrated in 3 sections (A), (B) and (C) in order to visualize 34 western blots in a proper way. Number 1 corresponds to raw hazelnut flour; numbers 2, 3, 4 and 5 correspond to AC 121°C 15 mins., 121°C 30 mins., 138°C 15 mins., and 138°C 30 mins., respectively. Numbers 6, 7, 8 and 9 correspond to HHP 300Mba, 400Mba, 500Mba and 600Mba respectively. Sera 1 and 2 correspond to non allergic patients to hazelnut (negative controls). (Note: (i) The film-exposition - after the ECL incubation- took longer than 24 h to visualize the bands of the negative controls: sera 1 and 2) (ii) The film-exposition - after the ECL incubation- took between 5 s and 1 min. according to the different sera tested in order to visualize the bands: from serum 3 until 17) (MW: molecular weight, AC: autoclave process, HHP: high pressure process, KDa: kilodalton).