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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: J Immunol. 2012 Sep 12;189(8):4079–4087. doi: 10.4049/jimmunol.1201061

FIGURE 8.

FIGURE 8

CD4+ and CD8+ T cells, and innate-like Thy1.2+CD3−CD4−CD8− γδTCR−cells, are major sources of IFN-γ and IL-17A during Mtb challenge of BCG-vaccinated/anti-IL10R-treated CBA/J mice. CBA/J mice were BCG-vaccinated with either concomitant anti-IL-10R mAb or isotype control mAb, and Mtb-challenged as described for Fig. 6. Lung cells were isolated at 112-days post challenge and were restimulated with PPD and anti-CD28 as described in Materials and Methods, with brefeldin-A added to the culture for the last 4 h. Intracellular staining was performed to determine the percentages of IFN-γ- and IL-17A-producing lymphoid cells by flow cytometry (A), and the total numbers of cytokine-positive cells in the lung were calculated using the total cell counts acquired from each individual mouse (B). Dot plots shown are concatenated from all individuals per group (A). Data shown is from a representative experiment of two timepoints with similar results (n = 5 per group). ns = not significant. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA.