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. Author manuscript; available in PMC: 2012 Oct 9.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Jul 22;425(2):244–249. doi: 10.1016/j.bbrc.2012.07.075

Figure 1. pmTOR is expressed at high levels in normal mouse and human glomeruli.

Figure 1

Kidneys were harvested from C57BL/6 mice, and immunofluorescence was performed on 3μm cryostat sections. Illustrated are low power (Panel A) and high power (Panel B) micrographs of the renal cortex showing intense expression of pmTOR within glomeruli and minimal expression on occasional tubules in extra-glomerular tissues. Panels C and D illustrate a similar pattern of expression of pmTOR in a human kidney transplant biopsy (Panel C) and in normal human kidney tissue (Panel D). In Panel E, glomeruli were isolated from C57BL/6 mice using magnetic beads (as described in Methods), and the expression of pmTOR, pS6 kinase and GAPDH was evaluated by Western blot analysis. Illustrated is the expression of pmTOR and pS6 kinase in the glomerular isolates (Glom) and in non-glomerular (Non-G) renal tissue.