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. 2012 Oct 9;7(10):e47194. doi: 10.1371/journal.pone.0047194

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) The diagram of RAcc-royalisin purified at UV A₂₈₀ nm from the recombinant E. coli. 1–2 (indicated by arrows): The outflow peak of the first injection samples; 3–4 : The outflow peak of the second injection samples; 5–10 : The elution peak of RAcc-royalisin. (B) The SDS-PAGE pattern of the purified RAcc-royalisin. Lane M: Protein marker; Lane 1–5: The elution peak products; Lane 6: The outflow peak products.