Table 1. Full-term development of embryos fertilized in vitro and cultured in a plastic bag without using an incubator.
| Treatment | Conc. of CO2 | Conc. of O2 | Culture medium | No. of cultured embryos | No. (%) of morulae/ blastocysts | No. of embryos transferred | No. (%) of embryos implanted | No. (%) of offspring | Mean body weight (g) | Mean placental weight (g) | Fertile* |
| Control | 5% | 5% | CZB | 88 | 87 (99) | 71 | 43 (60)a | 31 (44)a | 1.27±0.17 | 0.12±0.03 | Yes |
| KSOM | 89 | 87 (98) | 70 | 41 (59) | 30 (45) | 1.11±0.21a | 0.12±0.02 | Yes | |||
| Air-Oven | 0.03% | 20% | CZB | 100 | 98 (98) | 80 | 28 (35)b | 21 (26)b | 1.29±0.13 | 0.11±0.01a | Yes |
| KSOM | 100 | 83 (83) | 80 | 38 (48) | 25 (31)c | 1.27±0.15b | 0.11±0.03 | Yes | |||
| DeO2-Oven | Not controlled | 5% | CZB | 111 | 109 (98) | 109 | 76 (70)a | 45 (41)a | 1.29±0.25 | 0.11 ± 0.03a | Yes |
| KSOM | 123 | 113 (92) | 99 | 59 (60) | 48 (49)d | 1.27±0.16b | 0.12±0.02 | Yes | |||
| MixG-Oven | 5% | 5% | CZB | 110 | 109 (99) | 100 | 46 (46) | 37 (37) | 1.36±0.16 | 0.13±0.03b | Yes |
| KSOM | 136 | 135 (99) | 80 | 45 (56) | 30 (38) | 1.30±0.14b | 0.13±0.02 | Yes |
*
For fertility testing, three pairs were selected from each group at random.
a
vs. b, c vs. d; values with different superscript letters are significantly different (P<0.05 by χ2 tests or Student's t-tests).