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. 2012 Oct 9;7(10):e47512. doi: 10.1371/journal.pone.0047512

Table 1. Full-term development of embryos fertilized in vitro and cultured in a plastic bag without using an incubator.

Treatment Conc. of CO2 Conc. of O2 Culture medium No. of cultured embryos No. (%) of morulae/ blastocysts No. of embryos transferred No. (%) of embryos implanted No. (%) of offspring Mean body weight (g) Mean placental weight (g) Fertile*
Control 5% 5% CZB 88 87 (99) 71 43 (60)a 31 (44)a 1.27±0.17 0.12±0.03 Yes
KSOM 89 87 (98) 70 41 (59) 30 (45) 1.11±0.21a 0.12±0.02 Yes
Air-Oven 0.03% 20% CZB 100 98 (98) 80 28 (35)b 21 (26)b 1.29±0.13 0.11±0.01a Yes
KSOM 100 83 (83) 80 38 (48) 25 (31)c 1.27±0.15b 0.11±0.03 Yes
DeO2-Oven Not controlled 5% CZB 111 109 (98) 109 76 (70)a 45 (41)a 1.29±0.25 0.11 ± 0.03a Yes
KSOM 123 113 (92) 99 59 (60) 48 (49)d 1.27±0.16b 0.12±0.02 Yes
MixG-Oven 5% 5% CZB 110 109 (99) 100 46 (46) 37 (37) 1.36±0.16 0.13±0.03b Yes
KSOM 136 135 (99) 80 45 (56) 30 (38) 1.30±0.14b 0.13±0.02 Yes
*

For fertility testing, three pairs were selected from each group at random.

a

vs. b, c vs. d; values with different superscript letters are significantly different (P<0.05 by χ2 tests or Student's t-tests).