Table 3. Preimplantation development of inbred strain (C57BL/6) mouse embryos cultured in a plastic bag without using an incubator.
Treatment | Controlled gas conc. | Conc. of CO2 | Conc. of O2 | Humidity | No. of cultured embryos | No. (%) of 2- cell embryos | No. (%) of morulae/ blastocysts |
Control | Mixture* | 5% | 5% | 100% | 30 | 30 (100) | 30 (100) |
DeO2-WP | Air with deoxidizing agent | Not controlled | 5% | Not controlled | 100 | 99 (99) | 93 (93) |
5% CO2, 5% O2 and 90% N2.