Figure 5. C57/BL6 iPSC-derived GFP⁺ Flt1⁺/Flt4⁺ CPCs transplanted into strain-matched hearts engraft and robustly differentiate into CMs and smooth muscle cells.

Post-injection immunofluorescence imaging of GFP⁺ Flt1⁺/Flt4⁺ CPCs transplanted into the left ventricle of C57/BL6 mice demonstrates in vivo engraftment and robust differentiation into CMs and smooth muscle cells. (A & B) Immunostaining of GFP⁺ Flt1⁺/Flt4⁺ injected myocardial sections for CMs: (a,d) single channels for GFP and (b,e) single channels for TropC with (c,f) merged images. (C) Immunostaining of GFP⁺ Flt1⁺/Flt4⁺ injected myocardial sections for smooth muscle cells: (g) single channel for GFP and (h) α-SMA along with (i) merged images.