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. 2012 Oct 9;7(10):e46934. doi: 10.1371/journal.pone.0046934

Figure 1. Pax6 deficiency led to decrease of PC1/3 C-terminal cleavage and activity in mice.

Figure 1

A, expression of 87 kDa and 66 kDa PC1/3 in isolated islets in three operations as measured by western blot, each blot show the islets protein from several mice in wild type (from left to right, n = 7, 3, 3) versus Pax6 mutant mice (n = 9, 3, 3). 60 µg islets protein of wild-type and Pax6 mutant mice was used. B, ratio of densitometry scanning of 87 kDa and 66 kDa PC1/3 bands is shown (n = 3). C, Pax6 mutant mice (Pax6m/+) exhibited lowered activity of PC1/3. Islets protein of wild-type (n = 6) and Pax6 mutant mice (n = 6) with equivalent PC1/3 normalized by western blot (the upper row) were used to determine PC1/3 activity in the presence of 400 µM fluorogenic substrate. Wild type: white bars, Pax6m/+: black bars. A representative experiment is shown; the experiment was repeated three times. Each value represents the mean ± SE. *p<0.05, **p<0.01.