Figure 6. Pax6 directly down-regulated Pcsk1n expression.

A, Wild-type Pax6 (white bars), but not the truncated Pax6 (black bars), down-regulated Pcsk1n promoter activity as analyzed by using a luciferase report system in HEK293 cells (n = 3). B, in MIN6 cells transfected with Pax6 siRNA (black bars) but not scrambled siRNA (white bars) led to an increase in luciferase activity of the −1999Luc promoter construct (indicated as Pcsk1n promoter) (n = 3). Results of empty pGL₃ vectors are showed as a negative control. Decreased Pax6 production in cells after transfecting Pax6 siRNA was confirmed by western blotting analysis (upper panel). Each value represents the mean ± SE. *p<0.05, **p<0.01.