Figure 4. DCC is expressed by a subpopulation of GnRH neurites extending from POA explants in vitro.

Example images from dual-label immunocytochemistry to detect GnRH (green, left panel), DCC (red, middle panel), and two colors merged (right panel) performed on explants that had been grown for 2 DIV. Note that three different classes of neurites were visualized: GnRH⁺/DCC⁺ (arrows), GnRH⁺/DCC^- (double arrowheads), and GnRH^−/DCC⁺ (arrowheads). Scale bar, 20 µm.