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. 2012 Sep 24;63(16):5859–5872. doi: 10.1093/jxb/ers235

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Native-PAGE/activity staining of debranching enzyme (DBE), branching enzyme (BE), and starch synthase (SS) in developing endosperm of transgenic and wild-type rice. The ISA (isoamylase), PUL (pullulanase), PHO (phosphorylase), BEI, BEIIa, BEIIb, SSIIIa, and SSI activity bands are indicated by arrowheads. Crude extracts were prepared by adding 3 vols of grinding solution per fresh weight of the developing endosperm. The volume of crude extract applied to the native gels in DBE, BE, and SS were 5, 2, and 8 µl, respectively.