Table 4.
Summary of genotypes, activity levels of SSIIa, ISA, and BEIIb, and phenotypes in lines used in this study and transgenic rice lines containing soluble and insoluble α-glucans
| Lines | References | Genotypes | Activity levels | Dehulled grain weighta (mg) | Insoluble α-glucan (%) | ΔH by DSCb (%) | Crystallinity by X-rayb (%) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SSIIa | ISA (hetero) | ISA (homo) | BEIIb | |||||||||||||||||
| T65 | This study | WT (japonica) | – | + | ++ | ++ | 19.4 | 97.4 | 100 | 100 (A type) | ||||||||||
| IR36 | This study | WT (indica) | + | + | ++ | ++ | 16.8 | 97.0 | 100 | 100 (A type) | ||||||||||
| EM914 | This study | isa1 (japonica) | – | – | – | + | 11.3 | 3.1 | 0 | 0 | ||||||||||
| #20 | This study | OE-SSIIa I / c isa1 | + | – | – | + | 9.3 | 65.6 | 10 | 20 (B type) | ||||||||||
| #G5-1 | Fujita et al. (2003) | DR-ISA1/WT | – | ± | ± | ++ | 21.0 | 83.8 | 50 | 30 (A type) | ||||||||||
| #1-1 | Tanaka et al. (2004) | OE-BEIIb/be2b | – | + | ++ | +++ | 7.7 | 89.2 | 20 | 10 (A type) | ||||||||||
| Wx a :ISA2 | Utsumi et al. (2011) | OE-ISA2/WT | – | +++ | – | + | 10.2 | 92.6 | 0 | 5 (?) | ||||||||||
a Mean of 20 seeds.
b Percentage of the wild type.
c Background
OE, overexpression; DR, down-regulation.