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. Author manuscript; available in PMC: 2012 Oct 9.
Published in final edited form as: Nat Struct Mol Biol. 2008 Sep 21;15(10):1076–1083. doi: 10.1038/nsmb.1494

Figure 2.

Figure 2

Effects of SMK box riboswitch mutagenesis on SAM binding and apparent SAM binding constant (Kd) determination. (a) Effects of mutation (with s.d.) on SAM binding by size-exclusion filtration assay, as described24,35. (b) SAM and SAH Kd determination by competition binding assay. Binding curves are shown for the full-length E. faecalis metK RNA (open diamonds, Kd = 0.85 µM) and the SMK6 crystallization construct (filled diamonds, Kd = 0.57 µM). Unlabeled SAH showed no competition at a > 100-fold excess of SAH over SAM.