Figure 2. Detection and quantification of OXLAM profile by ESI/LC/MS/MS.
Individual isomers of HODEs and oxoODEs formed by LA oxidation were quantified with a single injection. Lipid extracts were resolved by HPLC and monitored online by ESI/LC/MS/MS [3]. Abbreviations: HODE: hydroxy-octadecadenoic acid; oxoODE, oxo-octadecadenoic acid; 15-HETE-d8, internal standard.