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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Immunol. 2012 Sep 14;53(3):283–294. doi: 10.1016/j.molimm.2012.08.020

Figure 4. Response of n3.L2 and M2 hybridomas to Hb(64-76) APLs.

Figure 4

A. All 20 amino acids were substituted individually into each of the 4 TCR contact positions in the Hb(64-76) peptide (P2, P3, P5 and P8). Wildtype Hb(64-76) residues are noted under the label for each position. APLs that induced IL-2 production are shaded. Each APL was tested in triplicate up to 100μM in at least 3 independent experiments. B. Stimulation of IL-2 production by n3.L2 (closed square) and M2 (open triangle) with M69 (left) and D70 (right) as examples of differences in APL response. Mean + SEM is presented for the full dose curve which is representative of 4 independent experiments.