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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Immunol. 2012 Sep 14;53(3):283–294. doi: 10.1016/j.molimm.2012.08.020

Figure 7. M2 CD4 T cells are hyporesponsive to Hb(64-76).

Figure 7

A. Rag1−/− CAb+ CD4+ cells isolated from n3.L2 (closed squares) and M2 (open triangle) mice were cultured in triplicate overnight with irradiated B6.K splenocytes pulsed with indicated concentrations of purified Hb(64-76) peptide. After 18-20 hrs, IL-2 production was assayed by ELISA. Dose curves are mean + SEM of triplicate samples from 3 independent experiments. B. The same cells assayed for IL-2 production in panel A were maintained in culture for an additional 72 hrs to measure proliferation of isolated n3.L2 (closed squares) and M2 (open triangle) cells by 3H incorporation. Dose curves are mean + SEM of triplicate samples from 3 independent experiments. C. Levels of costimulatory and inhibitory molecules on the surface of naive CAb+ CD4+ cells from the spleen of n3.L2 (black line) and M2 (filled histogram) TCR transgenic mice. Representative plots from 5 mice in 3 independent experiments.