Table 2.
Evaluation of iPSC-derived BMEC TEER.
| Co-cultured cell | Co-culture mediuma | Maximum TEER (Ωxcm2)b |
|---|---|---|
| IMR90-4-derived BMECs | ||
| Monoculture | 1% PDS | 222 ± 51 |
| HEKs | 1% PDS | 236 ± 23 |
| HEKs | 10% FBS | 364 ± 53 |
| HEKs (optimized)c | 10% FBS | 899 ± 132 |
| Astrocytes | 1% PDS | 412 ± 38 |
| Astrocytes | 10% FBS | 696 ± 8 |
| Astrocytes (optimized)c | 10% FBS | 1450 ± 140 |
| DF19-9-11T-derived BMECs | ||
| Astrocytes (optimized)c | 10% FBS | 777 ± 112 |
a
Refers to serum component of culture media. Full media details found in Materials and Methods. 1% PDS = EC medium.
b
Measured at approximately 24 hours of co-culture. TEER is expressed as mean ± standard deviation measured from triplicate filters.
c
Refers to optimized BMEC subculture density to reduce nonspecific cell adherence and debris accumulation.