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. 2012 Oct 10;3:228. doi: 10.3389/fpls.2012.00228

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Copyright © Chaiwongsar, Strohm, Su and Krysan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Seed production and embryo development of map3kε1^-/-;map3kε2^-/-;alc-YFP-MAP3Kε1 plants. (A) Mean number of seeds produced per silique for plants grown in the absence of exogenous ethanol treatment. Error bars indicate standard error. n ≥150 siliques for each genotype. (B) Siliques of 3-week-old plants grown in the absence of exogenous ethanol treatment. Shriveled seeds are indicated by white asterisks. (C,D) Microscopic analysis of embryo development. (C) Images at 2 days after flowering (DAF). (D) Images at 5 DAF. Images were obtained by capturing the autofluorescence of fixed embryos using a confocal microscope.