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. 2012 Sep 28;7:687–696. doi: 10.2147/COPD.S34560

Figure 2.

Figure 2

A representative western blot of Prolastin®.

Notes: Equal amounts of Prolastin were analyzed by 7% weight/volume acrylamide nondenaturing polyacrylamide gel electrophoresis without and with sodium dodecyl sulfate, blotted and probed with antihuman α1-antitrypsin antibodies (goat antihuman α1-antitrypsin antibody, dilution 1:10000 and rabbit antigoat immunoglobulin G peroxidase-conjugated secondary antibody, dilution 1:20000). Blots were developed with Amersham® ECL Western Blot analysis system (GE Healthcare, Little Chalfont, United Kingdom).

Abbreviations: PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.