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Impact of substitutions of Gly-56, -59,-93 and -97 with Ala on the function and expression of PCFT. A: [3H]MTX (0.5 μM) influx was assessed at pH 5.5 over 1 min. Data are means ± SE from 3 separate experiments. B: expression of the PCFT mutants in the crude membrane preparation. The blot is representative of 3 independent analyses. The white line on the image indicates lanes that have been repositioned.
