Fig. 6.

Cross-linking of human PCFT with 1,1-methanediyl bismethanethiosulfonate (MTS-1-MTS). A: identification of a Cys residue cross-linked in human PCFT. WT- and Cysless-PCFT, along with PCFT mutants in which one of the native Cys residues was replaced with serine, were subjected to the cross-link reaction. Molecular size markers are indicated at left. B: Gly mutants, along with WT-PCFT and Cysless-PCFT, were subjected to the cross-link reaction. The blots are representative of 2 independent experiments.