Skip to main content
. 2012 Jul 18;303(6):C616–C624. doi: 10.1152/ajpcell.00149.2012

Fig. 4.

Fig. 4.

PODXL is a functional selectin ligand expressed by SW1990 metastatic pancreatic tumor cells. Effect of PODXL-KD on E- (A and B) and L-selectin (C) binding was assessed via parallel-plate flow chamber. A: scramble control and PODXL-KD SW1990 cells were flowed overimmobilized E-selectin (10 μg/ml) at shear stresses of 1 and 0.5 dyn/cm2. Micrographs were captured after 2 min, and bound cell singlets are indicated by dashed circles. Scale bar = 100 μm. B and C: scramble control (black bars) or PODXL-KD (white bars) cell singlet binding to E- and L-selectin (10 and 20 μg/ml, respectively) was quantified over n = 3 experiments. Bars represent average binding compared with scramble control cells, error bars indicate SE. *P < 0.05. Control cell binding to E-selectin at 1 and 0.5 dyn/cm2 was 51 ± 4 and 87 ± 7 static cells/mm2, respectively. Control cell binding to L-selectin at 1 and 0.5 dyn/cm2 was 150 ± 15 and 339 ± 43 swift tethering events/mm2, respectively.