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. 2012 Sep 11;11:322. doi: 10.1186/1475-2875-11-322

Table 1.

Primer Sequences for PfATP6 codons 263, 623 and 769

Genomic region Primer Name Primer Sequence
PfATP6 Codon 263
263P1
5'-CTCCCGCTGATGCAAG-3'
263P2
5'-CCATGAATTGGATCTG-3'
263 N1
5'-CAGTTGACAAATATGCTG-3'
263WT
5'-GATAATTGTTGACCAAATA-3'
263 M
5'-GATAATTGTTGACCAAATG-3'
PfATP6 Codon 623 and 769 ATP1
5'-GGGTATCAACAAATTC-3'
ATP2
5'-CTTCAAATTCTCTTCC-3'
623WT
5'-TATACTACAGCTCAGGC-3'
623 M
5'-TATACTACAGCTCAGGA-3'
623 N2
5'-ACACTCATAAGTTTCC-3'
769 N1
5'-ACTTAGCTTTGCTTATAAAAACTTAA-3'
769 N2 5'-AATTATCCTTTTCATCATCTCC-3'

Original primer sequences from P. Cravo (personal communication).