Table 1.
Genomic region | Primer Name | Primer Sequence |
---|---|---|
PfATP6 Codon 263 |
263P1 |
5'-CTCCCGCTGATGCAAG-3' |
263P2 |
5'-CCATGAATTGGATCTG-3' |
|
263 N1 |
5'-CAGTTGACAAATATGCTG-3' |
|
263WT |
5'-GATAATTGTTGACCAAATA-3' |
|
263 M |
5'-GATAATTGTTGACCAAATG-3' |
|
PfATP6 Codon 623 and 769 | ATP1 |
5'-GGGTATCAACAAATTC-3' |
ATP2 |
5'-CTTCAAATTCTCTTCC-3' |
|
623WT |
5'-TATACTACAGCTCAGGC-3' |
|
623 M |
5'-TATACTACAGCTCAGGA-3' |
|
623 N2 |
5'-ACACTCATAAGTTTCC-3' |
|
769 N1 |
5'-ACTTAGCTTTGCTTATAAAAACTTAA-3' |
|
769 N2 | 5'-AATTATCCTTTTCATCATCTCC-3' |
Original primer sequences from P. Cravo (personal communication).