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. Author manuscript; available in PMC: 2012 Oct 10.
Published in final edited form as: Nat Immunol. 2011 Nov 20;13(2):144–151. doi: 10.1038/ni.2187

Figure 2.

Figure 2

IL-22 producing-NK like cells are markedly reduced in AHR-/- mice. (a, b) siLP (a) and PP (b) cells were isolated from AHR-/- or WT mice and stimulated with IL-23. Intracellular content of IL-22 was determined in CD3-CD19-NKp46+ cells. Numbers above bracketed lines indicate percent of IL-22+ cells (mean ± s.d.) Data are representative of 2 independent experiments (n=3). (c) Cells were isolated from siLP and PP of WT and AHR-/- mice, stained for CD3, CD19, NKp46 and NK1.1 and analyzed by flow cytometry. Numbers above outlined areas indicate percent cells gated on CD3-CD19- cells. (d) Quantification of NKp46+NK1.1-, NKp46+NK1.1lo, and NKp46+NK1.1+ subsets in siLP and PP of AHR-/- and WT mice (y-axis = percent cells). Data points represent individual mice and indicate frequency of cells gated on CD3-CD19- cells. (e,f) Representative flow cytometry analysis of cells isolated from the siLP (e) and PP (f) of AHR-/- and WT animals. Cells are gated on CD3-CD19-CD4- and numbers in the quadrants represent percent cells in each.