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. Author manuscript; available in PMC: 2012 Oct 10.
Published in final edited form as: Nat Immunol. 2011 Nov 20;13(2):144–151. doi: 10.1038/ni.2187

Figure 3.

Figure 3

AHR-/- mice lack CD4+LTi-like ILC as well as CP and ILF in the small intestine. (a) Flow cytometry analysis of cells isolated from the PP and siLP of AhR-/- or WT mice. Plots show cells gated on CD3-CD19-NKp46- and numbers above outlined areas indicate percent cells in each. Data are representative of 2 independent experiments (n=4). (b) Intracellular IL-22 expression in IL-23 stimulated LTi-like ILC from siLP or PP (gated on CD3-CD19-NKp46-CD4+). Numbers above bracketed lines represent percent IL-22+ cells (mean ± s.e.m.). Data are representative of 2 independent experiments (n=4). (c,d,e,f,g,h) Immunohistochemistry to identify cryptopatch CD90+c-kit+ clusters as well as CD11c+ and B220+ cells associated with CP and ILF in the small intestine. (c,d) CD90 in green, NKp46 in red in WT (c) and AHR-/- mice (d). (e,f) CD90 in green, c-kit in red in WT (e) and AHR-/- mice (f). (g,h) CD11c in green and B220 in red in WT (g) and AHR-/- mice (h). Scale bar for (c to h) 50μm. (i) Quantification of total CD90+ clusters along the small intestine (SI). (j) Frequency of PP along the small intestine (SI). ND – not detected. NS – not significant. (k) Quantification of the number of domes per PP along the whole intestine.