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. Author manuscript; available in PMC: 2012 Oct 10.
Published in final edited form as: Nat Immunol. 2011 Nov 20;13(2):144–151. doi: 10.1038/ni.2187

Figure 6.

Figure 6

IL-22 producing NK-like cells are reduced in RBP-Jκf/f × Cre-Vav1 mice. (a) siLp from RBP-Jκf/f and RBP-Jκ-/- mice were harvested and analyzed by FACS for NKp46+ILC. The cells in the top panel are gated on CD3-CD19-. Intracellular staining for IL-22 on the bottom panels show cells gated on CD3-CD19-NKp46+ cells. (b) siLP from RBP-Jκf/f and RBP-Jκ-/- mice were harvested and analyzed by FACS for CD4+ LTi-like ILC. Cells are gated on CD3-CD19-. (c) Quantification of the frequency of NKp46+ and LTi-like ILC from (a and b). (d) Immunohistochemistry to identify CD11c+ and B220+ cells associated with CP and ILF in the small intestine. CD11c in green and B220 in red in RBP-Jκf/f and RBP-Jκ-/- mice. Scale bar 50μm. (e) Quantification of total CD90+ clusters along the small intestine (SI).