Figure 5. No influence of genetic susceptibility on the effect of Treg depletion during allergen challenge.

(A) DEREG-C57BL/6 mice were sensitized to OVA with Alum on day 0, day 14 and day 21. One week post last sensitization DT was administered to mice i.p. Mice were challenged with 30 µg OVA via intra-nasal route for two consecutive days and analyzed 24 hr post last challenge. (B) Total cellular infiltration in BAL was determined by live cell counting using trypan blue exclusion dye. (C) Differential cell count was performed on Diff-Quik stained cytospots made from BAL cells. 200 cells were counted from random fields under light microscope in a single blinded manner. Data shown is a representative of three individual experiments using 3–6 mice in each group. Each dot represents data from individual mouse. Horizontal bar indicate mean value. Mann Whitney test was used to determine statistical significance. n.s.  =  non-significant.