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Genotyping of nonobese diabetic (NOD) Toll-like receptor (TLR)4-deficient (TLR4−/−; NOD.TLR4-deficient) mice and the development of diabetes. A: genotyping of NOD.TLR4-deficient mice shown using wild-type (WT) primers on the left and primers for the TLR4 knockout (TLR4KO) mutation on the right. Two WT mice (WT1 and WT2) and two NOD.TLR4-deficient mice (KO1 and KO2) are shown. B: development of diabetes in WT NOD mice compared with NOD.TLR4-deficient mice. F, female; M, male.
