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- mRNA. GFP-SRE- gene transcription was induced in wild-type, vts1Δ and eap1Δ cells with galactose and then shut off with glucose and reporter mRNA levels were assayed at the times indicated after transcriptional shutoff by Northern blot. The results of at least two independent experiments were quantitated and normalized using the levels of SCR1 RNA and graphed with error bars representing standard deviation.
