Figure 5. Eap1p stimulates transcript decapping.

Total RNA was isolated from the indicated cells expressing the GFP-SRE⁺ reporter during transcriptional pulse-chase experiments 16 min after transcriptional shut off with glucose. These samples were subjected to immunoprecipitation using an antibody that recognizes the 5′ cap and equivalent amounts of RNA from starting material (T), immunoprecipitated material (P) and the supernatant from the immunoprecipitates (S) were assayed for GFP-SRE⁺ mRNA and SCR1 RNA levels via Northern blots. The results of three independent experiments were quantitated and the average percentage of material in each sample along with the standard deviation are indicated. Note that GFP-SRE⁺ is significantly enriched in immunoprecipitates from both TetO₇-DCP2 cells treated with doxycycline and eap1Δ cells as determined by a t test (P<0.01) while this is not the case in immunoprecipitates from xrn1Δ cells.