Figure 7. The Eap1p/eIF4E interaction is required for efficient decay of Vts1p target mRNAs.

eap1Δ cells were rescued with either wild-type Eap1p-HA or a mutant version of Eap1p that is defective in its ability to interact with eIF4E (Eap1p^mt-HA) and the stability of GFP-SRE⁺ mRNA was assessed in these cells using a transcriptional pulse-chase approach and Northern blot (A) as described in Figure 1. Western blot (B) was used to compare the expression of wild-type Eap1p-HA to that of Eap1p^mt-HA using the PSTAIR protein as a loading control. The results of at least 3 independent experiments were quantitated and normalized using the levels of SCR1 RNA and graphed with error bars representing standard deviation (C). Data from wild-type and eap1Δ cells is from Figure 1A.