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. 2012 Jun 29;303(5):H605–H618. doi: 10.1152/ajpheart.00366.2012

Fig. 5.

Fig. 5.

Apelin-13 increases survival and angiogenic growth factor expression in ischemic hearts. A: representative results from Western blot and densitometric analyses of myocardial Akt phosphorylation. Phosphorylated (p-)Akt was significantly reduced in mouse hearts subjected to ischemia for 24 h and 14 days compared with sham control hearts. Treatment with apelin-13 significantly prevented ischemia-induced downregulation of Akt phosphorylation and increased levels over sham control values at both time points (n = 4 mice/group). *P < 0.05. B: representative results from Western blot and densitometric analyses of myocardial endothelial nitric oxide synthase (eNOS) phosphorylation. p-eNOS was significantly reduced in mouse hearts subjected to ischemia for 24 h and 14 days compared with sham control hearts. Treatment with apelin-13 significantly prevented and increased levels over sham control levels of ischemia-induced downregulation of eNOS phosphorylation. (n = 4 mice/group). *P < 0.05. C: representative results from Western blot and densitometry analyses of myocardial VEGF protein expression at 24 h and 14 days after myocardial ischemia. VEGF expression was significantly increased in ischemic control mice compared with sham control mice at 24 h and 14 days. Treatment with apelin-13 resulted in a significant increase in VEGF expression compared with control mice subjected to myocardial ischemia for 24 h and 14 days and increased levels over sham controls (n = 4 mice/group). *P < 0.05. D: representative results from Western blot and densitometric analyses of Notch3 expression. Notch3 expression was significantly increased in ischemic control mice compared with sham control mice at 14 days. Treatment with apelin-13 resulted in significant increases in Notch3 expression compared with ischemic control mice at 14 days (n = 4 mice/group). *P < 0.05. E: representative results from Western blot and densitometric analyses of jagged1 expression. Jagged1 expression was similar in sham control mice at 24 h and 14 days post-MI. Treatment with apelin-13 resulted in a significant increase in jagged1 expression compared with ischemic control mice at 24 h and 14 days post-MI (n = 4 mice/group). *P < 0.05.