Table 2.
Analysis of mRNA expression of NF-κB-dependent inflammatory cytokines in lung homogenates from WT and Glrx1−/− mice subjected to sensitization and challenge with OVA
| Balb/C |
Glrx1−/− |
|||
|---|---|---|---|---|
| Relative Expression | Alum/OVA | OVA/OVA | Alum/OVA | OVA/OVA |
| 3 Chall. +48 h | ||||
| CCL-20 | 1.0 ± 0.3 | 3.8 ± 0.5* | 1.4 ± 0.2 | 1.9 ± 0.3† |
| IL-6 | 1.0 ± 0.2 | 1.5 ± 0.4 | 0.4 ± 0.1 | 1.4 ± 0.3 |
| RANTES | 1.0 ± 0.1 | 0.8 ± 0.1 | 1.0 ± 0.2 | 1.0 ± 0.1 |
| KC | 1.0 ± 0.1 | 7.6 ± 1.2* | 0.9 ± 0.2 | 3.6 ± 0.9† |
| IL-5 | 1.0 ± 0.3 | 9.4 ± 2.8* | 0.9 ± 0.4 | 1.1 ± 0.5† |
| 6 Chall. +48 h | ||||
| CCL-20 | 1.0 ± 0.3 | 2.4 ± 0.7* | 0.6 ± 0.1 | 2.4 ± 0.6 |
| IL-6 | 1.0 ± 0.1 | 4.8 ± 1.1* | 0.6 ± 0.2 | 2.1 ± 0.7 |
| RANTES | 1.0 ± 0.2 | 9.3 ± 2.2* | 1.7 ± 0.5 | 4.1 ± 0.7 |
| KC | 1.0 ± 0.2 | 2.4 ± 1.2 | 2.1 ± 0.8 | 1.3 ± 0.5 |
| IL-5 | 1.0 ± 0.1 | 6.2 ± 1.5* | 2.1 ± 1.6 | 9.8 ± 2.0* |
| 6 Chall. +7d | ||||
| CCL-20 | 1.0 ± 0.4 | 1.8 ± 0.4 | 0.4 ± 0.1 | 1.7 ± 0.6* |
| IL-6 | 1.0 ± 0.3 | 0.7 ± 0.3 | 0.5 ± 0.2 | 1.4 ± 1.0 |
| RANTES | 1.0 ± 0.1 | 0.4 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.1 |
| KC | 1.0 ± 0.1 | 2.5 ± 1.1* | 0.4 ± 0.1 | 1.1 ± 0.2 |
| IL-5 | 1.0 ± 0.2 | 4.4 ± 1.0* | 0.8 ± 0.1 | 3.8 ± 0.6* |
CCL-20, IL-6, RANTES, KC, and IL-5 mRNA from whole lung homogenates of OVA/OVA or alum/OVA mice were analyzed by real-time PCR analyses. Results were normalized to the housekeeping gene cyclophilin. CCL-20, chemokine (C-C motif) ligand-20; RANTES, regulated on activation normal T-expressed and presumably secreted; KC, CXCL1 (neutrophil-activating protein). Data are expressed as fold increases in expression compared with WT alum/OVA controls.
*
P < 0.05 (ANOVA) compared with respective alum/OVA control groups.
†
P < 0.05 (ANOVA) compared with WT OVA/OVA groups.