Skip to main content
. 2012 Jun 20;303(5):F757–F765. doi: 10.1152/ajprenal.00470.2011

Fig. 1.

Fig. 1.

Morphological changes and expression profiles of heat shock protein (HSP)47 and extracellular matrix (ECM) proteins in the kidneys of rats subjected to unilateral ureteral obstruction (UUO). A: kidney sections stained with hematoxylin and eosin (HE; a and b) and Masson's trichrome (c and d) showed notable tubular dilatation and expansion of the interstitial compartment due to fibrosis in UUO rats (b and d) compared with control rats (a and c). B: semiquantitative score indicating marked glomerular damage (1) and tubulointerstitial damage (2) in the renal cortex of rats with UUO. C: real-time PCR analyses revealed significant increases in the mRNA expression of HSP47, collagen type I (Col.I), collagen type IV (Col.IV), fibronectin (FN), and plasminogen activator inhibitor (PAI)-1 in the kidney cortexes of UUO rats compared with sham-operated control rats (n = 6, P < 0.01). D: significant increases in the expression of HSP47 and FN in tubular and interstitial compartments of the kidneys in UUO rats (b and d) compared with control rats (a and c) as assessed by immunohistochemistry. Also, Col.I expression was noted to be remarkably increased in the kidney of rats subjected to UUO (F) compared with control rats (E). E: Western blot analyses revealed increased protein expression of HSP47, Col.I, and FN in the kidneys of the UUO group. F: densitometric analysis of Western blot bands of HSP47, Col.1, and FN confirmed the increased expression of the respective proteins (n = 4). *P < 0.01 vs. the control group.