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Kidney adenosine levels during renal ischemia and after reperfusion. Kidney adenosine levels were measured by HPLC. Kidneys were snap frozen in liquid nitrogen at the indicated times (n = 3–4 kidneys/time point). Kidney adenosine rapidly increased within 5 min after renal ischemia, and this increase was maintained during ischemia. However, kidney adenosine decreased to preischemic levels 30 min after reperfusion. *P < 0.01 vs. 0 min.
