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. 2012 Jul 3;303(5):F721–F732. doi: 10.1152/ajprenal.00157.2012

Fig. 6.

Fig. 6.

PD-81723 reduces apoptosis in human renal proximal tubule (HK-2) cells. HK-2 cell apoptosis was induced by TNF-α (20 ng/ml) and cycloheximide (CHX; 10 μg/ml). A: representative poly(ADP-ribose) polymerase (PARP) and caspase-3 fragmentation as indexes of HK-2 cell apoptosis (representative images from 6 experiments). PD-81723 (10 μM) or vehicle (1% DMSO) was applied 30 min before the induction of apoptosis, and cells were harvested 16 h later. The selective A1AR antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX; 100 nM) prevented this reduction in HK-2 cell apoptosis. B: densitometric quantifications of cleaved PARP (n = 6) and caspase-3 (n = 6) in HK-2 cells. Values are means ± SE. *P < 0.05 vs. vehicle; #P < 0.05 vs. PD-81723. C: representative gel image demonstrating DNA laddering as another index of apoptosis in HK-2 cells. PD-81723 (10 μM) applied 30 min before the induction of apoptosis reduced laddering in HK-2 cells. DPCPX (100 nM) again prevented this reduction in HK-2 cell apoptosis. Photographs are representative of 4 independent experiments.