Airway function was detected and BAL fluid and serum were collected and evaluated by ELISA. A, RL and Cdyn values were obtained in response to increasing concentrations of inhaled MCh, as described in material and methods. Data represented mean±SEM (n = 20 in each group). *p<0.05. B, Cellular composition of BAL fluid. C, Cytokine levels in BAL fluid and serum. D, Eotaxin levels in BAL fluid and serum. IgE levels in serum. Total, total cells; Epi, epithelium; Mac, macrophages; Lym, lymphocytes; Neu, neutrophils; Eos, eosinophils. Date represent the mean±SEM (n = 20 in each group). *p<0.05, **p<0.01 significant differences comparing asthmatic group and control group.