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. 2012 Oct 10;7(10):e47288. doi: 10.1371/journal.pone.0047288

Figure 9. Cytokine production from asthmatic naïve CD4+T cells pretreated with or without GSI.

Figure 9

CD4+T cells pretreated with or without GSI and transfected with Lfng cDNA. All the groups, including control group, asthmatic group, asthmatic/DMSO (asthmatic CD4+T treated with DMSO as negative control), asthmatic/GSI (asthmatic CD4+T treated with GSI), asthmatic/Lfng (asthmatic CD4+T treated with Lfng vector) and asthmatic/GSI+Lfng (asthmatic CD4+T treated with GSI and Lfng vector) cells were stimulated by anti-CD3/anti-CD28 antibody and cultured for 3 days. The culture supernatants were collected to detect the IL-4, IL-5, IFN-γ, IL-12 levels by ELISA. *p<0.05. The results are from one representative experiment of three independent experiments. Lfng overexpression almost had the same effect as GSI blockage on Th2 cytokine promotion (IL-4, IL-5) but had a greater effect on Th1 cytokines (IFN-γ, IL-12) than GSI treatment.