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Kidneys from rats that were injected subcutaneously with either vehicle or vasopressin 45 min before death were perfusion fixed with 4% paraformaldehyde and then embedded in paraffin sectioned into 4-μm slices. Tissue slices were stained for UT-A1 (green). Slices also treated with the nuclear stain DAPI (blue). Images were collected from the papilla for the kidney with a ×40 objective. Light microscopy was performed with a Nikon Microphot-FX fluorescence microscope.
