Fig. 5.

Molecular analysis of the association between MARCKS and PIPS. Overlay binding assay showing purified GST-MARCKS (A) and a purified GST-MARCKS mutant (B) fusion protein, but not GST (C) each bind directly to various phospholipids in vitro, but with different affinities. The format for the PIP strip assays is the same as presented in Fig. 2. The sequences for the native MARCKS and mutant MARCKS constructs used to perform the PIP strip assays are given in the previous figure. The white dots present in various spots are due to burnout of the signal of the film.