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Statistical analysis of Fluorescence resonance energy transfer (FRET) efficiency as determined by acceptor photobleaching in Xenopus 2F3 cells. Xenopus 2F3 cells were transfected with the indicated constructs, fixed, and mounted on microscope coverslips for imaging. FRET determination is shown for images from 6 independent cells. YFP, yellow fluorescent protein; CYF, cyan fluorescent protein. Values are means ± SE (*P < 0.05).
