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NO donors reduce cAMP in the CNT. CNTs were incubated for 20 min in the presence of the NO donor (Z)-1-N-(3-aminopropyl)-N-(3-ammoniopropyl)amino]diazen-1-ium-1,2-diolate (DPTA; 150 μM) or vehicle and then assayed for cAMP content. Tubules isolated from each mouse were treated and analyzed as a pair. Triangles show the effect of DPTA in tubules from each mouse. Solid circles show means ± SE of these experiments.
