Fig. 2.

AMPK Thr172 phosphorylation and AMPK levels are reduced in pancreatic acinar cell lysates (Triton X-100) after cerulein treatment. A: phosphorylation of the AMPK activating site (Thr172) decreases when isolated rat pancreatic acini are treated with supraphysiological cerulein (100 nM) over a 90-min time period. Immunoblots show the protein levels of AMPK-α (B) and AMPK-β (C) subunits decrease over time when acinar cells are treated with cerulein, whereas levels of PKA (D) do not. Immunoblots shown are representative of at least 3 similar but separate experiments. Lower graph illustrates densitometry from immunoblots. *P < 0.05 vs. CER time t = 0; n ≥ 3.